TABLE 4.

Clinical staphylococcal isolates with dissenting or ambiguous results in identification

Isolate16S rDNA-based identificationBiochemical identification
RIDOM (% similarity)aGenBank (% similarity)bID 32 Staph (T index; %ID)cVITEK 2 (T index; selectivity)c
M01S. arlettae (100.0)S. arlettae (100.0)S. klosii (0.65; 63.7), S. saprophyticus (0.27; 33.4), S. xylosus (0.29; 2.6), S. warneri (0.00; 0.2)S. kloosii (0.37; good)
M02S. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. aureus (0.96; 99.9)S. aureus (0.97; excellent)
M03S. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. aureus (0.63; 99.9)S. aureus (1.0; excellent)
M04S. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. aureus (0.52; 93.0)S. aureus (0.65; very good)
M05dS. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. cohnii subsp. cohnii (0.23; 64.7), S. caprae (0.21; 30.6), S. hominis 2e (0.0; 3.2)S. hyicus (0.42; poor), S. chromogenes (0.33; poor)
M06S. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. aureus (0.72; 99.9)S. aureus (0.86; excellent)
M07dS. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. aureus (0.72; 99.9)S. aureus (0.25; good)
M08S. haemolyticus (98.5)S. haemolyticus (98.5)S. haemolyticus (0.45; 76.9), S. warneri (0.37; 22.4), S. hominis (0.04; 0.6)S. haemolyticus (0.89; excellent)
M10S. capitis subsp. S. capitis (100.0)S. capitis (100.0), S. epidermidis (100.0), S. caprae (100.0)S. capitis (0.82; 99.9)S. capitis (0.59; poor), Kocuria rosea (0.50; poor), S. cohnii subsp. cohnii (0.49; poor)
M12dS. epidermidis (100.0)S. epidermidis (100.00)S. epidermidis 1e (0.54; 83.3)Kocuria varians (0.44; good)
M13dS. capitis subsp. capitis (99.8)S. capitis (99.8), S. epidermidis (99.8), S. caprae (99.8)Kocuria rosea (0.95; 81.5)S. chromogenes (0.49; poor), S. auricularis (0.43; poor)
M14S. caprae (99.8)S. caprae (99.8), S. capitis (99.8), S. arlettae (99.8)S. caprae (0.58; 99.6)S. chromogenes (0.44; good)
M15S. caprae (100.0)S. caprae (100.0), S. capitis (100.0), S. arlettae (100.0)S. caprae (0.22; 97.4)S. chromogenes (0.36; poor), S. aureus (0.35; poor), S. warneri (0.29; poor)
M17S. cohnii subsp. cohnii (100.0)S. cohnii (100.0), S. saprophyticus (100.0)S. cohnii subsp. cohnii (0.33; 99.3)S. cohnii subsp. cohnii (0.56; poor), S. saprophyticus (0.54; poor)
M18S. cohnii subsp. cohnii (100.0)S. cohnii (100.0), S. saprophyticus (100.0)Micrococcus lylae (0.52; 96.9)S. cohnii subsp. urealyticus (0.77; excellent)
M20S. saprophyticus subsp. bovis (100.0)S. cohnii (99.8), S. succinus (99.8)S. saprophyticus (1.00; 99.9)S. saprophyticus (1.00; excellent)
M25dS. epidermidis (100.0)S. epidermidis (100.0)S. epidermidis 2e (0.62; 86.1)Kocuria varians (0.24; poor)
M26S. equorum subsp. equorum (100.0), S. equorum subsp. linens (100.0)S. equorum (100.0), S. equorum subsp. linens (100.0)S. equorum (0.31; 97.6)S. xylosus (0.44; good)
M27S. felis (100.0)S. felis (100.0)S. intermedius (0.31; 48.6), S. chromogenes (0.32; 25.9), S. xylosus (0.43; 15.4), S. simulans (0.29; 10.1), S. aureus (0.00; 0.1)S. chromogenes (0.78; poor), S. schleiferi (0.70; poor)
M33S. intermedius (100.0), S. delphini (100.0)S. intermedius (100.0), S. delphini (100.0)S. intermedius (0.99; 99.7)S. intermedius (0.66; poor), S. chromogenes (0.61; poor)
M34S. intermedius (100.0), S. delphini (100.0)S. intermedius (100.0), S. delphini (100.0)S. intermedius (0.46; 95.4)S. intermedius (0.66; poor), S. chromogenes (0.61; poor)
M35S. intermedius (100.0), S. delphini (100.0)S. intermedius (100.0), S. delphini (100.0)S. intermedius (0.67; 98.0)S. intermedius (0.75; excellent)
M39S. saprophyticus subsp. bovis (100.0)S. cohnii (99.8), S. succinus (99.8)S. saprophyticus (0.83; 99.9)S. saprophyticus (0.91; excellent)
M40S. aureus subsp. aureus (100.0), S. aureus subsp. anaerobius (100.0)S. aureus subsp. aureus (100.0), S. aureus (100.0), S. haemolyticus (100.0)S. aureus (0.69; 99.3)S. aureus (0.50; very good)
M46S. simulans (100.0)S. simulans (100.0)S. simulans (0.73; 98.6)S. haemolyticus (0.48; poor), S. simulans (0.46; poor)
M47S. hominis subsp. novobiosepticus (100.0)S. hominis (100.0)S. hominis 1e (0.21; 65.9), S. warneri (0.18; 17.0), S. epidermidis 2 (0.15; 10.9)S. capitis (0.86; excellent)
M48S. felis (100.0)S. felis (100.0)S. xylosus (0.58; 60.8), S. intermedius (0.30; 22.6), S. simulans (0.36; 14.8), S. chromogenes (0.16; 1.7), S. aureus (0.00; 0.1)S. simulans (0.60; poor), S. chromogenes (0.53; poor)
M49S. felis (100.0)S. felis (100.0)S. simulans (0.65; 83.3)S. chromogenes (0.53; poor), S. schleiferi (0.45; poor)
M51S. warneri (100.0)S. warneri (100.0)S. warneri (0.76; 99.9)S. warneri (0.46; poor), S. haemolyticus (0.46; poor), S. kloosii (0.39; poor)
M53S. equorum subsp. equorum (100.0), S. equorum subsp. linens (100.0)S. equorum (100.0), S. equorum subsp. linens (100.0)S. equorum (0.68; 66.0), S. xylosus (0.74; 34.0), S. sciuri (0.03; 0.1)S. xylosus (0.88; excellent)
M55S. xylosus (99.35), S. saprophyticus subsp. saprophyticus (99.35)S. xylosus (100.0)S. xylosus (0.89; 99.2)S. xylosus (0.90; excellent)
  • a Percent similarity compared to the 16S rDNA sequence of the type strain of the staphylococcal taxon.

  • b Results of redundant database entries as well as of entries without taxonomic classification were not included.

  • c In the case of identification with low discrimination below acceptable values as given by the biochemical identification kits, i.e., a percent identification accuracy (%ID) of <80.0 (as specified by ID 32 Staph) and poor selectivity (as specified by VITEK 2), all results are listed; otherwise, only results with the highest %ID and best selectivity, respectively, are shown.

  • d Isolate exhibiting the SCV phenotype.

  • e The numeral identifies the biotype.