TABLE 1.

Conventional axenic methods of inoculation performed in our laboratory for all specimens

Sample type(s)MediumAtmosphereLength of incubationTemp of incubation (°C)
Cerebrospinal fluidPVX chocolate agara,eAerobicbAt least 10 days37
CNA Columbia blood agarc,eAerobicb37
Columbia blood agarAnaerobic37
Columbia blood agare,iAerobicb37
5% sheep blood agar tubes for Mycobacterium spp.fAerobicb3 months37
Biopsy (cardiac valve, lymph node, bone marrow, or skin, but not lung) specimen, abscess, fluidPVX chocolate agar for Francisella spp.AerobicbAt least 10 days37
CNA Columbia blood agarAerobicb37
Columbia blood agar for Bartonella sp.Aerobicb20 days37
Columbia blood agarAnaerobic37
5% sheep blood agar tubes for Mycobacterium spp.3 months37j
Lung biopsy specimenCNA Columbia blood agarAerobicbAt least 20 days37
PVX chocolate agarAerobicb
Columbia blood agarAnaerobic37
5% sheep blood agar tubes for Mycobacterium spp.Aerobic3 months
MacConkey agareAerobic
BCYEd for Legionella spp.gAerobicb
± Regan Lowe medium for Bordetella pertussisgAerobic
Blood cultureAerobic bottlehAerobicAt least 5 days; 20 days for fastidious pathogens such as Brucella spp. and Bartonella spp.37
Anaerobic bottlehAnaerobicAt least 5 days37
  • a Chocolate agar enriched with polyvitex.

  • b With 5% atmosphere of CO2.

  • c CNA, colistin and nalidixic acid and 5% sheep blood agar.

  • d BCYE, buffered charcoal-yeast extract.

  • e bio-Mérieux (Marcy l'Etoile, France).

  • f Bio Technologie Appliquée (Dinan, France).

  • g Oxoid Ltd. (London, United Kingdom).

  • h BACTEC 9240 system (BBL Microbiology Systems, Cockeysville, Md.).

  • i For fastidious pathogens.

  • j 30°C for skin samples.