TABLE 1.

Oligonucleotide primer sequences used in PCR assaysa

GenePrimer sequence (5′-3′)Amplicon size (bp)
ctxA (forward)5′-CTCAGACGGGATTTGTTAGGCACG-3′
ctxA (reverse)5′-TCTATCTCTGTAGCCCCTATTACG-3′308
rstR1 (forward)5′-CTTCTCATCAGCAAAGCCTCCATC-3′500
rstR2 (forward)5′-GCACCATGATTTAAGATGCTC-3′500
rstR3 (forward)5′-CTGTAAATCTCTTCAATCCTAGG-3′∼300
rstR4 (forward)5′-GTTAACGCTTCAAGCCTG-3′400
rstA3 (reverse)5′-TCGAGTTGTAATTCATCAAGAGTG-3′
  • a Primers were for the detection of rstR and ctxA genes in V. cholerae O1 and O139 strains isolated from hospitalized patients in Matlab, Bangladesh.