TABLE 2.

Virus gene detection of serum samples from confirmed dengue patients by TaqMan RT-PCR and conventional RT-PCR

Ct range of TaqMan RT-PCR (cycles)Detection by:
TaqMan RT-PCRConventional RT-PCR
Type specificCross-reactive
<2510/10 (100)a10/10 (100)9/9 (100)
25-29.97/7 (100)6/7 (86)6/6 (100)
30-34.97/7 (100)6/6 (100)7/7 (100)
35-39.96/6 (100)3/4 (75)3/6 (50)
≥405/5 (100)2/4 (50)1/5 (20)
Total35/35 (100)27/31 (87)26/33 (79)
  • a Positive samples/number of tested samples (%).