TABLE 1.

Comparison of biochemical characteristics of “M. massiliense” and closely related mycobacterial speciesa

CharacteristicPresence (+) or absence (−) of various characteristics in:
M. massilienseM. abscessusM. chelonaeM. immunogenum
Growth at 24°C++++
Growth at 30°C++++
Growth at 37°C++++
Growth at 42°C
Growth at 45°C
Pigmentation
Arylsulfatase (3 days)++++
Catalase++++
Semiquantitative catalase++++
5% NaCl tolerance (30°C)++
MacConkey (without crystal violet)++++
Degradation of p-aminosalicylate
Iron uptake
Nitrate reductase++
Pyrazinamidase+++±
Pyrrolidonyl arylamidase++++
Phosphatase alkaline++++
β-Glucuronidase+
β-Galactosidase++
α-Glucosidase++++
N-Acetyl-β-glucosaminidase+
Esculin++++
Urease
Gelatinase++
Arginine dihydrolase
Lysine decarboxylase
Ornithine decarboxylase
Citrate+
H2O production
Tryptophane desaminase++
Indol production+
Acetoin production++++
Glucose
Ribose
Xylose
Mannitol
Maltose
Lactose
Saccharose
Glycogen
Inositol
Sorbitol
Rhamnose
Meliobiose
Amygdaline
Arabinose
  • a Results were reproducible over triplicate testing. Colony morphology was rough/smooth for all four species.