TABLE 1.

Sensitivity and reproducibility of the 18S screening real-time PCR and the Plasmodium species-specific real-time PCR when tested with a single target

TargetProbeMean Ct at dilutiona:
103 copies102 copies101 copies100 copies (% of isolates)
P. falciparum Plasprobe29.1 ± 1.033.1 ± 0.637 ± 0.740.3 ± 1.5 (100)
Falcprobe29.3 ± 0.532.7 ± 0.636.1 ± 138.9 ± 0.8 (66)
P. vivax Plasprobe28.3 ± 0.631.9 ± 0.736.7 ± 1.238.6 ± 0.3 (66)
Vivprobe30.2 ± 0.233.7 ± 0.437.6 ± 0.540.2 ± 0.4 (83)
P. malariae Plasprobe28.8 ± 0.532.8 ± 0.436.3 ± 0.239.1 ± 0.6 (83)
Malaprobe28.2 ± 0.532 ± 0.435 ± 0.438.4 ± 1.8 (50)
P. ovale Plasprobe29.6 ± 1.133.1 ± 1.436.8 ± 0.540.1 ± 1.4 (83)
Ovaprobe28.7 ± 0.932.4 ± 1.135.6 ± 1.539.3 ± 1.1 (66)
P. ovale (new)Plasprobe29.9 ± 2.233.2 ± 2.236.5 ± 1.640.0 ± 0.5 (66)
Ovaprobe29.8 ± 1.033.3 ± 1.036.9 ± 0.839.6 ± 1.1 (100)
  • a Mean values and standard deviations are based on two experiments, each done in triplicate.