TABLE 3.

Dideoxyoligonucleotide primers used for P. aeruginosa MLST

Locus and functionPrimer sequence (5′ to 3′)Amplicon size (bp)
ForwardReverse
acsA
    AmplificationACCTGGTGTACGCCTCGCTGACGACATAGATGCCCTGCCCCTTGAT842
    SequencingGCCACACCTACATCGTCTATGTGGACAACCTCGGCAACCT390
aroE
    AmplificationTGGGGCTATGACTGGAAACCTAACCCGGTTTTGTGATTCCTACA825
    SequencingATGTCACCGTGCCGTTCAAGTGAAGGCAGTCGGTTCCTTG495
guaA
    AmplificationCGGCCTCGACGTGTGGATGAGAACGCCTGGCTGGTCTTGTGGTA940
    SequencingAGGTCGGTTCCTCCAAGGTCTCAAGTCGCACCACAACGTC372
mutL
    AmplificationCCAGATCGCCGCCGGTGAGGTGCAGGGTGCCATAGAGGAAGTC940
    SequencingAGAAGACCGAGTTCGACCATATGACTTCCTCTATGGCACC441
nuoD
    AmplificationACCGCCACCCGTACTGTCTCGCCCATCTTGACCA1,042
    SequencingACGGCGAGAACGAGGACTACTTCACCTTCACCGACCGCCA366
ppsA
    AmplificationGGTCGCTCGGTCAAGGTAGTGGGGGTTCTCTTCTTCCGGCTCGTAG989
    SequencingGGTGACGACGGCAAGCTGTATCCTGTGCCGAAGGCGATAC369
trpE
    AmplificationGCGGCCCAGGGTCGTGAGCCCGGCGCTTGTTGATGGTT811
    SequencingTTCAACTTCGGCGACTTCCAGGTGTCCATGTTGCCGTTCC441