TABLE 2.

Primers used for amplification

Target(s)aPrimerSequencebProduct size (bp)Annealing temp (°C)Nucleotide positions (bp)cGenBank accession no.d
CTX-M group ICTXM1-F3GAC GAT GTC ACT GGC TGA GC49955416-435X92506
CTXM1-R2AGC CG C CGA CGC TAA TAC A914-896
CTX-M group IITOHO1-2FGCG ACC TGG TTA ACT ACA ATC C35155313-334X92507
TOHO1-1RCGG TAG TAT TGC CCT TAA GCC663-643
CTX-M group IIICTXM825FCGC TTT GCC ATG TGC AGC ACC30755475-495AF189721
CTXM825RGCT CAG TAC GAT CGA GCC781-764
CTX-M group IVCTXM914FGCT GGA GAA AAG CAG CGG AG474621857-1876AF252622
CTXM914RGTA AGC TGA CGC AAC GTC TG2330-2311
  • a Group I includes CTX-M-1, -3, -10 to -12, -15 (UOE-1), -22, -23, -28, -29, and -30. Group II includes CTX-M-2, -4 to -7, and -20 and Toho-1. Group III includes CTX-M-8. Group IV includes CTX-M-9, -13, -14, -16 to -19 and -21, and -27 and Toho-2. Finally, group V includes CTX-M-25 and -26.

  • b Sequence of primer as synthesized 5′ to 3′.

  • c Nucleotide position in base pairs for the GenBank accession number sequence.

  • d Accession number of the sequence used for primer design.