TABLE 1.

Initial screen of sPCR fragments with first-UTI (n = 88), pyelonephritis (n = 88), and rectal (n = 88) E. coli strains

sPCR fragment% UTI% Pyelonephritis% RectalPotential function (% amino acid identity)
S2T2-6526522Heat-resistant agglutinin (Hra) protein E. coli (91)
S2T1-91344415Putative transposase in Y. pestis (51)
S2T1-1156153No match
S2T2-9152011lifA gene (90)
S2T1-43363228Ribokinase RbsK from lactobacillus (38)
S2T1-5414147Hypothetical protein E. coli O157:H7 (25)
S2T1-9315136sugR ATP binding protein S. enterica serovar Typhimurium (86)
S2T2-11814867No match
S2T1-135151514Hypothetical lipoprotein H. pylori (50)
S2T1-4817225yscA protein B. subtilis (40)
S2T1-11621425Hypothetical protein in V. cholera (27)
S2T1-30201314Hyaluronan synthetase P. multicoda (66)
S2T1-86836182Hypothetical type II secretion protein E. coli EPEC strain (92)
S2T0-25083Predicted protein L. lactis (50)
S2T2-31aPutative integrase CP933U E. coli O157:H7 (97)
S2T1-89201114Unknown protein CP933M E. coli O157:H7 (51)
S2T2-481396Putative tail component CP933P E. coli O157:H7 (52)
S2T2-371977Putative integrase prophage CP933C E. coli O157:H7 (90)
  • a —, probe showed high degree of cross hybridization, possibly due to presence of related prophage sequences in most E. coli strains.