TABLE 3.

Familial distribution of ear canal phylotypes

ParameterValuea
IIIIIIIV
ABCDEFGHJK
A1A2A3B1aB1bB1cB1dB2C1C2D1D2E1E2F1F2F3G1H1aH1bH3H4J1K1
Phylotypeb
    A. otitis80977070556177381003179354112674759100979295
    C. otitidis131023028223340224525284245033
    S. auricularis3210619121111532360251
    C. auris44312
    Corynebacterium sp. strain 61720191
    S. epidermidis1113
    P. acnes1245
    Streptococcus gordonii31
    Fusobacterium periodonticum113
    Pseudomonas lanceolata121
    Enterobacter sp. strain B50912
    Uncultured bacterium strain BPC00911
    Enterobacter agglomerans11
    Tilletiara anomala435711621241
    M. obscurus fungus524
    Trhypochthanius tectorum163
    P. pubis fungus21
    Sclerotium sp.21
    Homo sapiens (mitochondrion)14
    Other1142613211341
No. of clones888793548989948484958990938773918480918189888387
PCR primerscUUUBUUUBUUUUUUUUUUBUUUUB
DNA extractiondSSSCSSSCSSSSSSSSSSSSSSSC
Collection siteeLLLLLLFLLLFFFFFFFFFFFFFL
Elapsed timef041213014
SexgMFFMFMFMFMFFFMMMFFMM
Ageh323253535656266613531231552839146657
  • a Column headers indicate family (roman numerals), household (letters), and individuals (letters plus numbers). Multiple samples for an individual are designated by lowercase letters.

  • b Nearest relative based on Blastn search. Data are presented as percentages of the numbers of clones screened for each library.

  • c U, Universal primers 515F-1391R; B, bacterial primers 8F-805R.

  • d S, simple boiling preparation; C, chemical, enzymatic, mechanical extraction of DNA.

  • e L, laboratory; F, field.

  • f Months elapsed from initial sampling for individuals sampled on multiple occasions.

  • g M, male; F, female.

  • h Age in years at time of sampling.