TABLE 1.

Programming of the LightCycler instrument

Program nameAnalysis modeNo. of cyclesTemp (°C)Time (s)Temperature transition rate (°C/s)Signal acquisition
DenatureNone19560020None
PCRQuantification45951020None
551020Single
72820None
Melt analysisMelt195020None
451020None
8000.2Continuous
CoolNone135020None