TABLE 1.

Phenotypic and genotypic properties of 132 MRSA isolates from Taipei, Taiwan, and Nanjing, China

ClaI-mecA::Tn554 typeaPFGE typeNo. of isolatesClonal typebAntimicrobial resistance (of the majority [>50%] of the isolates)cspaA typeMLST typeSTSCCmec type
Taiwan isolates
    III′::BA145III::B::A (n = 97; 82%)PEN, OXA, CIP, CLI, ERY, TET, GEN, SXTWGKAOMQ2-3-1-1-4-4-30241III
    III′::BA74
    IX::BA236WGKAOMQ2-3-1-1-4-4-30241IIIA
    IX::BA46
    IX::BA51
    IX::BA91
    IX::BA101
    XI::BA83WGKAOMQIII
    XIV::BA13XIV::B::APEN, OXA, CIP, CLI, ERY, TET, GEN, SXTWGKAOMQIII
    XIV::BA32
    XIV::BA61
    XIV::BA91
    X′::BA35X′::B::APEN, OXA, CIP, CLI, ERY, TET, GENWGKAOMQIIIA
    III′::AAA11III′::AA::APEN, OXA, CIP, CLI, ERY, TET, GEN, SXT, CHLWGKAOMQIII
    XI::BC2XI::B::CPEN, OXA, CIP, CLI, ERY, TET, GEN, SXT, CHL, RIFWGKAOMQ2-3-1-1-4-4-3239IIIA
    II::NHB11II::NH::BPEN, OXA, CLI, ERY, TET, GEN, CHLZDMDMOB19-23-15-2-19-20-1559IV
    II::NHB23
    II::ZZD2II::ZZ::DPEN, OXA, CLI, ERY, TET, GEN, CHL, RIFNTd3-32-1-1-4-4-381II variant
China isolates
    III′::BA117III::B::A (n = 10; 71%)PEN, OXA, CIP, CLI, ERY, TET, GEN, CHLWGKAOMQ2-3-1-1-4-4-3239III
    III′::BA121
    III′::BA162
    X′::BA131X′::B::APEN, OXA, CIP, CLI, ERY, TET, GEN, CHLWGKAOMQIIIA
    X′::BA142
    X′::BA151
  • a ClaI-mecA polymorphs and respective hybridization fragment sizes: III′, 1.9 and 4.5 kb; IX and XI, see reference 8; XIV, 1.9 and 4.3 kb; X′, 1.9 and 6 kb; II, see reference 13. Tn554 polymorphs: NH, no homology, lack of transposon; B and AA, see reference 13; ZZ, novel pattern (9.2, 7.1, 6.7, 6.1, and 4.1 kb).

  • b ClaI-mecA polymorphs III′, IX, and XI present minor variations compared to pattern III and were found to be genetically related (21). Therefore strains belonging to these mecA polymorphs were grouped under type III for the definition of clonal types.

  • c Abbreviations: PEN, penicillin; OXA, oxacillin; CIP, ciprofloxacin; CLI, clindamycin; ERY, erythromycin; TET, tetracycline; GEN, gentamicin; SXT, trimethoprim-sulfamethoxazole; CHL, chloramphenicol; RIF, rifampin.

  • d NT, there was no amplification with the set of primers used.