TABLE 1.

Primers for PCR deduced from M. genitalium 16S rRNA gene sequence used in initial evaluation

PrimerSequence
MG16-45F5′ TAC ATG CAA GTC GAT CGG AAG TAG C
MG16-181R5′ ACC CTT GCA GGT CCT TTC AAC TTT A
MG16-252R5′ CGT CAT TGC CTT GGT AGG CCA
MG16-411R5′ TTC TTC CCA AAT AAA AGA ACT TTA CAA TCA A
MG16-447R5′ AAA CTC CAG CCA TTG CCT GCT AG
MG16-455R5′ GGT ACA GTC AAA CTC CAG CCA TTG
MG16-439F5′ GAA TGA CTC TAG CAG GCA ATG GCT G
MG16-636R5′ TCC AAA ACT CCC TAC CAC ACT CTA GAC TG
MG16-1117R5′ TCC TCC AAT TTA CAT TAG CAG TCT CGT TAA
MG16-1204F5′ CAA TGG CCA ATA CAA ACA GTA GCC AA
MG16-1301R5′ CTG ATT CGC GAT TAC TAG TGA TTC CAG
MG16-1428R5′ ACC GGT GCT ATC CTT GAC ATG CA
MG16-1421R5′ GTG CTA TCC TTG ACA TGC ACT TCC AA
  • a Primer combinations MG16S-45F-MG16S-447R and MG16S-1204F-MG16S-1301R (underlined primers) were M. genitalium specific and used in the clinical evaluation.