TABLE 3.

Frequencies of and vancomycin MICs for subcolonies of passage-derived substrains grown on agar plates containing vancomycin at 4 mg/litera

StrainbNo. of colonies growing on plates containing vancomycin at:Frequency of subcolonies growing on BHI plates containing 4 mg of vancomycin/literMIC (mg/liter) of vancomycin for the one-step selected subcolonies from plates with vancomycin at 4 mg/liter
0 mg/liter4 mg/liter
Mu31.66 × 1082.95 × 1031.78 × 10−55
Mu502.09 × 1081.12 × 1080.538
Mu50-P352.24 × 1082.51 × 1031.12 × 10−57
MI-P841.42 × 1087.89 × 1045.56 × 10−47
NJ-P501.82 × 1081.32 × 1037.25 × 10−45
PC-P251.55 × 1083.02 × 1031.95 × 10−57
IL-P651.78 × 1081.23 × 1036.91 × 10−46
AMC11094-P841.51 × 1081.35 × 1058.94 × 10−47
99/3759-V-P841.59 × 1088.32 × 1045.23 × 10−46
99/3700-W-P251.16 × 1083.89 × 1043.35 × 10−45
LIM2-P301.57 × 1084.07 × 1042.59 × 10−46
98141-P652.38 × 1087.5 × 1043.15 × 10−45
28160-P651.31 × 1082.13 × 1051.64 × 10−35
BR1-P350.69 × 1087.96 × 1021.17 × 10−59
BR2-P250.76 × 10800
BR3-P600.28 × 1081.19 × 1024.25 × 10−67
BR4-P100.77 × 1084.11 × 1025.34 × 10−66
BR5-1-P300.83 × 1086.51 × 1027.84 × 10−68
  • a All cultivations of substrains were spreaded on BHI plates containing vancomycin at 0 and 4 mg/liter and incubated at 37°C for 24 h, and the MICs for subcolonies growing on vancomycin plates at 4 mg/liter were determined.

  • b The numbers after the suffix P indicate the days of passage.