TABLE 1.

PCR tests and assay variables

Target speciesReferenceTarget gene (amplicon size [bp])PrimersbVariable PCR parameters useda
MgCl2 concn (mM)Amt (U) of TaqPrimer annealing temp (°C)No. of cycles
C. jejuni 8 23S rRNA (710 or 810)F 5′-TAAAGTAAGTACCGAAGCTG-3′2.5c0.5c5827 (Pub)
R1 5′-GTAAATCCTAATACAAAGCT-3′
R2 5′-TAAATCCTAGTACGAAGCT-3′
4 Random (265)F 5′-ATC GGG CTG TTA TGA TGA TA-3′3.00.55735 (Pub)
R 5′-CAT ATC CAG AGC CTC TGG AT-3′
34 mapA (604)F 5′-ATG TTT AAA AAA TTT TTG-3′1.5 (Pub)2.5 (Pub)55 (Pub)35 (Pub)
R 5′-AAG TTC AGA GAT TAA ACT AG-3′
9 ceuE (793)F 5′-CCT GCT ACG GTG AAA GTT TTG C-3′2.50.5 (Pub)6530 (Pub)
R 5′-GAT CTT TTT GTT TTG TGC TGC-3′
32 Random (358)F 5′-GAA TGA AAT TTT AGA ATG GGG-3′2.50.5 (Pub)5735
R 5′-GAT ATG TAT GAT TTT ATC CT GC-3′
13 hipO (735)F 5′-GAA GAG GGT TTG GGT GGT-3′2.5 (Pub)0.566 (Pub)25 (Pub)
R 5′-AGC TAG CTT CGC ATA ATA ACT TG-3′
38 Randomd (773)F 5′-CA TCT TCC CTA GTC AAG CCT-3′2.00.56130
R 5′-AAG ATA TGG CTC TAG CAA GAC-3′
C. coli 8 23S rRNA (390)F 5′-TAT TCC AAT ACC AAC ATT AGT-3′1.0-2.50.5-3.054-6127-30
R 5′-TAA ATC CTA ATA CGA AGC G-3′
32 Random (258)F 5′-ATA TTT CCA AGC GCT ACT CCC C-3′2.50.5 (Pub)5735
R 5′-CAG GCA GTG TGA TAG TCA TGG G-3′
9 ceuE (894)F 5′-ATG AAA AAA TAT TTA GTT TTT GCA-3′3.00.5 (Pub)57 (Pub)30 (Pub)
R 5′-ATT TTA TTA TTT GTA GCA GCG-3′
13 Putative aspartokinase (500)F 5′-GGT ATG ATT TCT ACA AAG CGA G-3′2.5 (Pub)0.560 (Pub)25 (Pub)
R 5′-ATA AAA GAC TAT CGT CGC GTG-3′
38 Randomd (364)F 5′-AG GCA AGG GAG CCT TTA ATC-3′2.00.56130
R 5′-TAT CCC TAT CTA CAA ATT CGC-3′
  • a Pub, parameters as described in original publication.

  • b F, forward; R, reverse.

  • c Not stated by original authors; values shown are in-house parameters.

  • d Assay performed as a multiplex reaction for detection of both C. jejuni and C. coli, as originally described(38).