TABLE 2.

Interassay reproducibilities of the HCoV OC43 and HCoV 229E qRT-PCRsa

SampleDilutioncRNA copiesMean CTSDCV (%)
HCoV OC43 cRNA1 × 10−52.35 × 10815.960.020.14
1 × 10−62.35 × 10718.670.080.44
1 × 10−72.35 × 10622.220.020.08
1 × 10−82.35 × 10525.180.110.44
HCoV 229E cRNA1 × 10−52.26 × 10817.350.030.18
1 × 10−62.26 × 10720.820.030.13
1 × 10−72.26 × 10624.190.060.24
1 × 10−82.26 × 10527.760.070.24
  • a The mean values of CT, standard deviations (SDs), and coefficients of variation (CVs) were determined for three assay runs.