TABLE 3.

Statistical analysis of the quantification standards as evaluated in the SYBR Green assay

ParameterValue at indicated cell count/ml
10610510410310210110010−1
Avg Ct20.423.728.032.637.040.741.7
SD2.02.32.02.22.82.21.8
No. of Ct valuesa36454545403070
Ct intervals16.5-24.320.0-29.424.3-32.728.9-37.233.6-47.036.5-46.739.9-44.8
Significance P < 0.001 P < 0.001 P < 0.001 P < 0.001 P < 0.001 P = 0.18
  • a The maximum number of individual Ct values is 45 (3 tests at 15 samples each) for all concentrations except for the most concentrated (106/ml), which had 12 samples and thus a maximum of 36 individual Ct values.