TABLE 1.

Intra-assay variability of the HCoV OC43 and HCoV 229E qRT-PCRa

SamplecRNA copiesMean CTSDCV (%)
HCoV OC43 cRNA2.35 × 10815.980.050.33
2.35 × 10718.750.050.27
2.35 × 10622.240.030.13
2.35 × 10525.270.010.05
2.35 × 10428.820.060.21
2.35 × 10332.130.160.49
HCoV 229E cRNA2.26 × 10817.350.030.18
2.26 × 10720.790.030.13
2.26 × 10624.120.080.34
2.26 × 10527.820.180.63
2.26 × 10431.120.170.55
2.26 × 10334.250.150.43
  • a Each of the dilutions was assayed three times per run. The mean values of CT, standard deviations (SDs), and coefficients of variation (CVs) were calculated.