TABLE 1.

Results of amplification by consensus primer in the multiplex PCR assay, using three pairs of primers for adenoviruses (i.e., consensus primers and Ad3- and Ad7-specific primers) and original clinical specimens

Amplification by consensus primersNo. of specimens that previously grew an adenovirusaNo. of specimens that did not grow adenovirusbTotal no. of specimens
Positive1152c117
Negative12d5163
Total12753180
  • a Included specimens that grew Ad1 (n = 13), Ad2 (n = 19), Ad3 (n = 29), Ad4 (n = 8), Ad5 (n = 7), Ad6 (n = 4), Ad7 (n = 36), Ad11 (n = 3), and Ads of unusual types (n = 8).

  • b Included specimens that yielded no specific agents (n = 20), influenza type A (n = 2) and B (n = 1), respiratory syncytial virus group A or B (n = 8), parainfluenza virus types 1, 2 and 3 (n = 3), Streptococcus pneumoniae (n = 1), and Mycoplasma pneumoniae (n = 18).

  • c Included one specimen that yielded influenza virus type A and one specimen with no specific agents identified, and amplicons of consensus primers from these specimens were confirmed by sequencing analysis to belong to Ad subgroup C.

  • d Included specimens that grew Ad1 (n = 5), Ad2 (n = 1), Ad3 (n = 1), Ad5 (n = 3), Ad7 (n = 1), and Ads of unusual types (n = 1).