TABLE 6.

Conventional and real-time PCR results for specimens from suspected extraintestinal amebiasis (liver abscesses)

MethodologyNo. of liver aspirates specimens identified as:Total
E. histolyticaNegative
E. histolyticaNegative
Sequencinga504c9
Conventional PCRb4149
SYBR Green3249
LightCycler5049
TaqMan 15049
TaqMan 25049
  • a DNA sequencing analysis was performed only to assure the results and not for comparison purposes.

  • b Results from confirmatory diagnosis, performed directly upon reception of the specimen at CDC.

  • c Negative liver aspirates were positive for bacterial liver abscess by culture methods.