TABLE 3.

AFLP multilocus genotypes for C. neoformans var. gattii isolates from Renmarka

LocusResult for isolate:
R1 (A)R2 (B)R3 (C)R4 (D)R5 (E)R6 (F)R7 (G)R10 (H)R11 (I)R12 (J)R13 (K)R14 (L)R15 (M)R17 (N)R18 (O)R19 (P)R20 (Q)R21 (R)R22 (S)R23 (T)R24 (U)R25 (V)R26 (W)R27 (X)R28 (Y)R29 (Z)R30 (AA)
A011110000111111000011111110
B011111100111111110111111100
C011110000111111000111111100
D110000000100101001111110110
E001110000111000000011101000
F000000000000110000000010011
G011110001111111001011111100
H111100000011101000011111100
I111100000011001000011111111
J001111010000110000000000011
K000000100110001111000110100
L111110011111001111001111101
M111110111110111111001111100
N111101000011000000000000000
O001111010010110000100001011
P000000101100001111000110100
Q011111010011110000101001011
R001100000011100000010100000
S001110000011110000011001000
T100000000000011101000000000
U011110000111101000001111000
V001111010010110101101001011
W001111011110111111101111111
X011100000011000000001101000
Y011100000011000100001101000
Z001111000011110000111001010
AA001111000011110000111001010
AB001111111110111111101111110
AC011100000011000000011100000
ADb011111010011110000001001010
AE011100000111101000011111100
AF011100000011101000011100000
AGb011111010011110000000101010
AH101000101100001111110110101
AIb011111010011110000001001010
AJ011100000111101000011101100
AKb100000101100001111110110101
AL110111110110011001011111111
  • a The columns R1 to R30 are the 27 C. neoformans var. gattii isolates and the rows A to AL are the loci included in the analysis. After each isolate designation, the multilocus genotype is indicated in parentheses. Loci A to G were amplified by primers EcoRI-GT-MseI-GT, loci H to T were amplified by primers EcoRI-CA-MseI-GT, loci U to AB were amplified by primers EcoRI-CA-MseI-GA, loci AC to AH were amplified by primers EcoRI-TG-MseI-TG, and loci Al to AL were amplified by primers EcoRI-TG-MseI-CA. 1, amplified band present; O, amplified band absent.

  • b Locus was removed from the study when it was realized that it segregated the isolates according to their mating types.