TABLE 1.

Restriction enzymes, adaptors, and primers

Restriction enzymeRecognition siteAdaptor oligonucleotidesaPrimerPrimer name
XbaI5′T↓CTAGA5′CTAGTACTGGCAGACTCT5′AGAGTCTGCCAGTACTAGApx
AGATC↓T    3′ATGACCG5′AGAGTCTGCCAGTACTAGpxn
NheI5′G↓CTAGC5′AGAGTCTGCCAGTACTAGCpn
CGATC↓G5′AGAGTCTGCCAGTACTAGCGpn-g
PstI5′CTGCA↓G5′CTCGTAGACTGCGTACATGCA5′GACTGCGTACATGCAGGpst-g
G↓ACGTC    3′CATCTGACGCATGT5′GACTGCGTACATGCAGAGpst-ag
5′GACTGCGTACATGCAGATpst-at
EcoRI5′G↓AATTC5′AATTGGTACGCAGTCTAC5′GTAGACTGCGTACCAATTCpeco
CTTAA↓G    3′CCATGCGTCAGATGCTC
  • a One of the oligonucleotides of each adaptor was phophorylated at its appropriate 5′ site. Adaptors were chosen from references 4, 8, and 15.