TABLE 1.

PCR assays tested including gene targets, primer designations, annealing and cycling conditions, limits of detection, and specificity results

Target genePrimer pairAnnealing temp (°C)No. of cyclesDetection limitaSpecificitybReference
RrBhEc
16Sge9-ge1055402.57
16Sec12-ec948, 523, 372.57
Nestedge9-ge105530
16Sehr521-ehr74760500.25+++24
16Sehr521-ehr79055400.25+16
16Sper1-per245402.5+13
16Sger3-ger45040UNNTNTNT13
16Sge3a-ge1055400.2519
Nestedge9-ge25530
groEhs1-hs648, 523, 370.25 weak+26
Nestedhs43-hs455530
ankla6-la155402.527
msp2msp2-3f-msp2-3r55400.25Massung, 1999c
100 kDas7f-s7r554025 weakNTNTNT25
130 kDas22f-s22r55402.5 weakNTNTNT25
Hsp-70b3f1-b3r554025NTNTNT25
  • a The estimated minimum number of A. phagocytophilum infected cells that could be detected per test sample. UN, could not be determined.

  • b Bacteria used for specificity testing: Rr, R. rickettsii strain Sheila Smith; Bh, B. henselae strain Houston-1; Ec, E. chaffeensis strain Arkansas. NT, not tested.

  • c R. F. Massung, presented at the EUWOG-ASR Joint Meeting, Marseille, France, 1999 (Program and Abstracts, p. 6), was subsequently published in reference 20.