TABLE 1.

Forward and reverse sequences of primers, based on sequences found in GenBank or those of published or unpublished primers

GenePrimer sequenceaReaction parametersbAmplicon size (bp)cReference or accession no.
arcAF: 5′-GAAGACGAGTTGGTAACACG-3′95°C for 1 min, 55°C for 2 min, 72°C for 3 min, 30 cycles68017
R: 5′-CTTCCAGATCACCGCAGAAGC-3′
aroEF: 5′-AAGGTGCGAATGTGACGGTG-3′95°C for 1 min, 57°C for 2 min, 72°C for 3 min, 28 cycles62017
R: 5′-AACTGGTTCTACGTCAGGCA-3′
dnaEF: 5′-GAG/TATGTGTGAGCTGTTTGC-3′94°C for 45 s, 45°C for 45 s, 72°C for 1 min, 30 cycles550P. Garg, personal communication
R: 5′-CGA/GATA/CACCGCTTTCGCCG-3′
mdhF: 5′-CAACTGCCTTCAGGTTCAGAA-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 30 cycles580AE005551
R: 5′-GCGTTCTGGATGCGTTTGGT-3′AP002564
gndF: 5′-GGCTTTAACTTCATCGGTAC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min 10 s, 30 cycles590AE005428
R: 5′-TCGCCGTAGTTCAGATCCCA-3′AP002559
gapAF: 5′-GATTACATGGCATACATGCTG-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min 10 s, 30 cycles535AE005401
R: 5′-CAGACGAACGGTCAGGTCAAC-3′AP002558
pgmF: 5′-CCG/TTCG/CCAG/TAACCCGCC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 35 cycles60013
R: 5′-TCA/GACA/GAACCATTTGAAA/G/TCC-3′
espAF: 5′-ATGGATACATCAAA/CTGG/CA/CAC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 35 cycles579AE005594
R: 5′-TTATTTACCAAGGGATATT-3′AP002566
ompAF: 5′-AGACAGCTATCGCGATTGC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 30 cycles691AE005286
R: 5′-GCTTTGTTGAAGTTGAACAC-3′AP002554
  • a F, forward; R, reverse.

  • b All reactions had an initial denaturation at 94°C for 4 min and a final extension at 72°C for 4 min.

  • c The sequenced DNA strands were typically shorter than the original PCR amplicon.