TABLE 1.

Forward and reverse sequences of primers, based on sequences found in GenBank or those of published or unpublished primers

GenePrimer sequenceaReaction parametersbAmplicon size (bp)cReference or accession no.
arcA F: 5′-GAAGACGAGTTGGTAACACG-3′95°C for 1 min, 55°C for 2 min, 72°C for 3 min, 30 cycles68017
R: 5′-CTTCCAGATCACCGCAGAAGC-3′
aroE F: 5′-AAGGTGCGAATGTGACGGTG-3′95°C for 1 min, 57°C for 2 min, 72°C for 3 min, 28 cycles62017
R: 5′-AACTGGTTCTACGTCAGGCA-3′
dnaE F: 5′-GAG/TATGTGTGAGCTGTTTGC-3′94°C for 45 s, 45°C for 45 s, 72°C for 1 min, 30 cycles550P. Garg, personal communication
R: 5′-CGA/GATA/CACCGCTTTCGCCG-3′
mdh F: 5′-CAACTGCCTTCAGGTTCAGAA-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 30 cycles580 AE005551
R: 5′-GCGTTCTGGATGCGTTTGGT-3′ AP002564
gnd F: 5′-GGCTTTAACTTCATCGGTAC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min 10 s, 30 cycles590 AE005428
R: 5′-TCGCCGTAGTTCAGATCCCA-3′ AP002559
gapA F: 5′-GATTACATGGCATACATGCTG-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min 10 s, 30 cycles535 AE005401
R: 5′-CAGACGAACGGTCAGGTCAAC-3′ AP002558
pgm F: 5′-CCG/TTCG/CCAG/TAACCCGCC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 35 cycles60013
R: 5′-TCA/GACA/GAACCATTTGAAA/G/TCC-3′
espA F: 5′-ATGGATACATCAAA/CTGG/CA/CAC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 35 cycles579 AE005594
R: 5′-TTATTTACCAAGGGATATT-3′ AP002566
ompA F: 5′-AGACAGCTATCGCGATTGC-3′94°C for 45 s, 50°C for 45 s, 72°C for 1 min, 30 cycles691 AE005286
R: 5′-GCTTTGTTGAAGTTGAACAC-3′ AP002554
  • a F, forward; R, reverse.

  • b All reactions had an initial denaturation at 94°C for 4 min and a final extension at 72°C for 4 min.

  • c The sequenced DNA strands were typically shorter than the original PCR amplicon.