TABLE 1.

Sensitivity and specificity of four assays for detection of Leptospira antibodies, calculated using two by two contingency tables

Assay% Sensitivity (95% CI)% Specificity (95% CI)
Paired seraa (n = 133)Acute-phase serab (n = 148)Convalescent-phase serac (n = 128)
ELISA86.5 (79.5-91.8)48.7 (40.4-57.0)75.0 (66.6-82.2)97.0 (95.4-98.2)
IHA79.0 (71.0-85.5)38.5 (30.6-46.9)67.2 (58.3-75.2)95.8 (93.9-97.2)
LDS93.2 (87.5-96.9)52.7 (44.3-61.0)83.6 (76.0-89.6)89.6 (86.9-91.8)
DST92.5 (86.6-96.3)50.0 (41.7-58.3)84.4 (76.9-90.2)98.8 (97.6-99.5)
MATd48.7 (40.4-57.0)93.8 (88.1-97.3)97.3 (95.8-98.4)
  • a For any assay, a case was considered positive if either the acute- or convalescent-specimen tested positive.

  • b Specimens collected within 14 days of onset of disease.

  • c Specimens collected >14 days after onset of disease.

  • d MAT was part of the case definition and defined most cases.