TABLE 1.

PCR primers used to amplify ddl, gdh, rpoB, sodA, and gtf gene sequences

Target genePrimer namePrimer sequenceaAnnealing temp (°C)Expected size (bp)
ddl ddl-F 5′-GCYATGGATAAAATYACRAC-3′ 50563
ddl-R 5′-CCACTGGKTRAARCCTGGCAGRGT-3′
gdh gdh-F 5′-CGTGGYGGCTAYTATGACC-3′ 50642
gdh-R 5′-CYTCRTCCCAGTGRCTRAARTTRG-3′
rpoB rpoB-F 5′-AARYTIGGMCCTGAAGAAAT-3′ 50742
rpoB-R 5′-TGIARTTTRTCATCAACCATGTG-3′
sodA sodA-F 5′-TRCAYCATGAYAARCACCAT-3′ 50438
sodA-R 5′-ARRTARTAMGCRTGYTCCCARACRTC-3′
gtf gtf_uni-F 5′-GAAACTGTTGATGGCTATTTGACAGC-3′ 50678
gtf_uni-R 5′-CATTGACATTATCCACCGCATCGACACG-3′
  • a I, inosine; Y, C or T; R, A or G; K, G or T; M, A or C.