TABLE 8.

Phylogenetic distribution of virulence-associated traits among 329 Escherichia coli isolates from women with cystitis or pyelonephritis and uninfected women

TraitaPrevalence of trait (no. [%] of isolates) within phylogenetic groupb:
A (n = 37)B1 (n = 23)B2 (n = 197)D (n = 72)
papA/C/EF/G2-3 (5-8)(***)0 (***)134-135 (68)***42 (58)
papG allele II2 (5)(***)0 (***)97 (49)***42 (58)**
papG allele III0 (***)0 (*)54 (27)***3 (4)(***)
sfa/focDE0 (***)1 (4)(*)76 (39)***1 (1)(***)
sfaS 01 (1)26 (13)***1 (1)(**)
focG 0 (**)1 (1)40 (20)***0 (***)
afa/draBC6 (16)031 (16)*3 (4)(*)
iha 7 (19)(**)0 (***)82 (42)43 (60)***
fimH 32 (86)(**)20 (87)(*)197 (100)***69 (96)
hlyD 2 (5)(***)1 (4)(**)90 (46)***11 (15)(***)
cnf1 0 (***)2 (9)71 (36)***1 (1)(***)
cdtB 01 (4)27 (13)***0 (***)
iroN 1 (3)(***)4 (17)89 (45)***7 (10)(***)
fyuA 14 (38)(***)6 (26)(***)195 (99)***41 (57)(***)
iutA 8 (22)(**)1 (4)(***)98 (50)44 (61)**
kpsM II9 (24)(***)5 (22)(***)184 (93)***61 (85)
K1 kpsM3 (8)(***)4 (17)82 (42)***15 (21)*
traT 12 (32)(***)7 (30)(***)148 (75)***51 (71)
ibeA 0 (**)3 (13)35 (18)**5 (7)
ompT 6 (16)(***)9 (39)(***)183 (93)***52 (72)
malX 2 (5)(***)1 (4)(***)190 (96)***19 (26)(***)
ExPEC2 (5)(***)0 (***)135 (69)***41 (57)
  • a Traits shown are those that yielded P values of <0.05 in both the initial screen for heterogeneity among groups and at least one pairwise comparison, including afa/dra (Dr-binding adhesins), cdtB (cytolethal distending toxin), cnfI (cytotoxic-necrotizing factor 1), fimH (type 1 fimbriae), focG (F1C fimbriae), fyuA (yersiniabactin siderophore system), hly (hemolysin), iha (adhesin-siderophore), ireA (siderophore receptor), ibeA (invasion of brain endothelium), iroN (siderophore receptor), iutA (aerobactin system), kpsM II (group 2 capsule), K1 (K1 kpsM II variant), K2 (K2 kpsM II variant), malX (pathogenicity island marker), ompT (outer membrane protease T), papA/C/EF/G (P fimbriae structural subunit, assembly, tip pilins, and adhesin), papG alleles II and III (P fimbriae adhesin variants), rfc (O4 lipopolysaccharide synthesis), sfa/foc (S and F1C fimbriae), sfaS (S fimbriae adhesin), and traT (serum resistance associated). Traits detected in ≥1 isolate each, but not significantly phylogenetically distributed, include (the percentage of the total of 329 isolates is indicated in parentheses) cvaC (colicin [microcin] V; 3%), gafD (G fimbriae; 0.6%), iss (increased serum survival; 4%), K2 kpsM II variant (4%), kpsM III (group 3 capsule; 2%), and rfc (O4 lipopolysaccharide synthesis; 2.4%); papG allele I and bmaE were not detected in any isolate.

  • b P values (by Fisher's exact test) are indicated by asterisks where P is <0.05. Symbols: *, P < 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Parentheses indicate negative associations.