TABLE 1.

Restriction enzymes, adaptors, and primersb

Restriction enzymeRecognition siteAdaptor oligonucleotidesaPrimer
SequenceName
XbaI5′-T↓CTAGA AGATC↓T5′-CTAGTACTGGCAGACTCT 3′-ATGACCG5′-AGAGTCTGCCAGTACTAGA 5′-AGAGTCTGCCAGTACTAGPx Pxn
5′-AGAGTCTGCCAGTACTAGCGPn-g
NheI5′-G↓CTAGC CGATC↓G5′-CTAGTACTGGCAGACTCT 3′-ATGACCG5′-AGAGTCTGCCAGTACTAGA 5′-AGAGTCTGCCAGTACTAGPx
5′-AGAGTCTGCCAGTACTAGCGPn-g
PstI5′-CTGCA↓G G↓ACGTC5′-CTCGTAGACTGCGTACATGCA 3′-CATCTGACGCATGT5′-GACTGCGTACATGCAGAG 5′-GACTGCGTACATGCAGATPst-ag Pst-at
EcoRI5′-G↓AATTC CTTAA↓G5′-AATTGGTACGCAGTCTAC 3′-CCATGCGTCAGATGCTC
  • a One of the oligonucleotides of each adaptor was phosphorylated at its appropriate 5′ site.

  • b Selective bases are indicated in boldface.