TABLE 2.

Results of LAMP assay detecting tcdB in DNA extracted from stool specimens and overnight culture in CMM and comparison to other tests

TcdB detectiona in stool specimen by cell cultureC. difficile cultureDirect detection of tcdB in stool specimen by:Detection of tcdB from overnight culture in CMM by LAMPNo. of stool specimens
TcdB detectionTcd type of isolateNested PCRLAMP
++A+B++++16
++A+B+++2
++A+B+++NDc13
Invalid+A+B++++1
++AB++++1
+A+B++++3
+A+B+++1
+AB1
NAb+++2
NA++5
NA++ND1
NA++2
NA+3
NA+ND1
NA10
NAND6
ND+A+B+++ND2
NDNA++ND2
NDNAND2
  • a +, positive; −, negative. Invalid, test result invalid because of atypical cytotoxic effect.

  • b NA, not applicable.

  • c ND, not done.