TABLE 2.

Identification of clinical yeast isolates by MALDI-TOF MS and conventional tests

SpeciesTotal no. of isolatesNo. of isolates identified with API ID 32C systemNo. of isolates identified by MALDI-TOF MSb
First runSecond runa
Candida albicans87858787
Candida glabrata52495252
Candida tropicalis35333535
Candida parapsilosis28262628
Candida metapsilosis1001
Candida krusei18181818
Candida kefyr9799
Candida (Clavispora) lusitaniae7467
Candida norvegensis6660 6
Candida rugosa1110 1
Candida dubliniensis2110 2
Candida lipolytica1111
Candida ciferrii1110 1
Candida pelliculosa1111
Candida guilliermondii1111
    Subtotal250234245240 250
Cryptococcus neoformans3333
Saccharomyces cerevisiae2122
Trichosporon cutaneum3330 3
Magnusiomyces/Blastoschizomyces capitatus2222
Galactomyces geotrichum/Geotrichum silvicola1000 1
Pichia kluyveri1000 1
Pichia fabianii/Pichia veronae2000 2
Pichia (Kodamaea) ohmeri1110 1
Cryptococcus curvatus1000 1
Pichia rhodanensis1000 1
    Subtotal1710117 → 17
    Total267244256247 → 267
  • a After repeating the test with the API ID 32C system for isolates with discrepant results.

  • b MALDI-TOF MS identification was performed in parallel on a Microflex LT spectrometer (Leipzig) and a Biflex III spectrometer (Bonn), and there were no discrepancies in the results. →, identified after integration into the database.