TABLE 2.

Primers used in this studya

Marker locusPrimer designationPrimer sequence
clfAClfA-F5′-GATTCTGACCCAGGTTCAGA
ClfA-R5′-CTGTATCTGGTAATGGTTCTTT
clfBClfB-F5′-ATGGTGATTCAGCAGTAAATCC
ClfB-R5′-CATTATTTGGTGGTGTAACTCTT
sdrSdrCDE-F5′-GTAACAATTACGGATCATGATG
SdrCDE-R5′-TACCTGTTTCTGGTAATGCTTT
spaSpa-F5′-AGCACCAAAAGAGGAAGACAA
Spa-R5′-GTTTAACGACATGTACTCCGT
sspSspA-F5′-ATCMATTTYGCMAAYGATGACCA
SspA-R5′-TTGTCTGAATTATTGTTATCGCC
  • a The primers were designed based on the completed and unfinished S. aureus genomes available from The Institute for Genomic Research (http://www.tigr.org) (COL strain), the University of Oklahoma (http://www.genome.ou.edu) (strain 8325), Sanger Institute (http://www.sanger.ac.uk) (strains MRSA252 and MSSA476), and Juntendo University (http://www.juntendo.ac.jp) (strains Mu50 and N315).