TABLE 1.

Detection of NLVs by using conventional RT-PCRs and real-time quantitative RT-PCR with 81 EM-positive stool specimens from 36 outbreaks of nonbacterial acute gastroenteritis

OutbreakStool codeConventional RT-PCRReal-time RT-PCRb
No.Mo-yrPlaceFor RdRpa (nested)For capsidb,c
1Jan.-97RestaurantU1d+GIIGII
U2d+GIIGII
2Feb.-97Food productU3dGIIGII
U4dGIIGII
3Oct.-97Nursery schoolU5d+GIIGII
U6d+GIIGII
U27+GIIGII
U28+GIIGII
4Nov.-97Nursing homeU7d+GIIGII
U8d+GIIGII
5Nov.-97Food productU9d+GIIGII
6Dec.-97RestaurantU29+GIIGII
7Dec.-97RestaurantU10dGIIGII
U11d+GIIGII
8Dec.-97RestaurantU12d+GIIGII
U13d+GIIGII
9Jan.-98RestaurantU30+GIIGII
U31+GIIGI + GII
10Jan.-98RestaurantU32d
11Jan.-98HotelU201d+GIIGII
U15d+GIIGII
12Feb.-98DormitoryU16d+GIIGII
U17d+GIIGII
13May-98SchoolU18d+GIIGII
U19d+GIIGII
U20d+GIIGII
U21d+GIIGII
14Dec.-98RestaurantU22d+GIIGII
U23d+GIIGII
U24d+GIIGII
15Dec.-98RestaurantU25dGII
U26dGII
16Mar.-99HotelKU4d+GIGI
KU6d+GIGI
KU7d+GIGI
17Apr.-99RestaurantKU8d+GIGI
KU9d+GIIGII
18Apr.-99RestaurantKU10dGIGI
19May-99SchoolKU17dGIIGII
20May-99SchoolKU24dGIGI
KU25GIGI
21Jun.-99HotelKU34d+GIIGII
22Jun.-99Food productKU44d+GIIGII/PICK>
23Oct.-99RestaurantKU45GII
24Oct.-99Nursery schoolKU62d+GII
KU63dGII
KU64dGII
KU66d+GII
25Nov.-99SchoolKU68d+GIIGII
26Nov.-99RestauranteKU80d+GI + GIIGI + GII
KU82d+GI + GIIGI + GII
KU83d+GI + GIIGI + GII
KU84d+GIIGII
27Dec.-99School partyKU85dGIIGII
KU88d+GIIGII
KU89d+GII
KU90+GIIGII
KU91+GIIGII
28Dec.-99SchoolKU93d+GIIGII
29Dec.-99RestaurantKU98d+GIIGII
KU99dGII
KU101d+GIIGII
30Dec.-99RestaurantKU105d+GI + GIIGI + GII
KU106+GI + GIIGI + GII
KU109d+GI + GIIGI + GII
KU111dGI + GIIGI + GII
KU112dGIGI
KU115dGIGI
31Jan.-00Food productKU5d+GII
32Jan.-00FamilyKU16d+GIIGII
33Jan.-00RestauranteKU18d+GIIGII
KU19d+GI + GIIGI + GII
KU26d+GIIGII
KU27dGIIGII
34Mar.-00RestaurantKU31d+GII
KU32d+GII
35Mar.-00RestauranteKU35d+GIIGII
KU36d+GI + GIIGI + GII
KU37d+GII
36Mar.-00HotelKU49d+GIIGII
KU53d+GIIGII
Total81626780
Detection rate (%)778399
  • a +, positive; −, negative.

  • b Method described in reference 31. Method described in reference 21.

  • c GI, GI detected; GII, GII detected; −, negative.

  • d Used for sequence analysis.

  • e From shellfish-related outbreak.