TABLE 1.

Oligonucleotides used to generate recombinant chlamydial fusion proteins

Organism and proteinOligonucleotide pairs (5′→3′)aExpression vector, cloning site(s)
C. pneumoniae
    CrpAGAGctcgagATGTCATCAAATCTACATCCpET-14b, XhoI
GATctcgagCTAAACGCGAGCTATTTTACT
    PorBTAggatccCTTTGACATGAATAGCAAGATGpET-14b, BamHI
TTggatccTCTAAAACTGCAGACCTGACGT
    OMP2AGctcgagATGTCCAAACTCATCAGACGAGpET-14b, XhoI
CTTctcgagTTAATACACGTGGGTATTTTC
    OMP3AAActcgagAAGAAAGCTGTTTTAATTGCTpET-14b, XhoI
CCActcgagTTACTGTTTGCATCTGCCATC
    MOMP-VD1GTGCGACGgaattcGCTTACGTGCTGGpGEX-2T, EcoRI
AGCTCCTgaattcCAGAAAACATCAAAGCG
    MOMP-VD2CTTTGATggatccTGTACTTTAGGAGCpGEX-2T, BamHI
AACTTTAGGTTTggatccTGCATATTGG
    MOMP-VD3CTTATGggatccGGTTGTGCAACTTTGGpGEX-2T, BamHI
GGATGTTATCAggatccAAAGTTGCTCG
    MOMP-VD4CCATACATTGgaattcAATGGTCTCGApGEX-2T, EcoRI
GAATCTgaattcACCAGATACGTGAGC
C. trachomatis
    CrpAAGGAATGTAActcgagAGCACTGTACCCGTpET-14b, XhoI
TAGAGCAATctcgagTCATTGGGTCTGATCC
    OMP2AAAATAActcgagCGAATAGGAGATCCTATGpET-14b, XhoI
CATCGATAAAActcgagATTAATAGATGTGTG
    MOMP-VD2ATCGTTTTGATggatccTGTACATTAGGAGpGEX-2T, BamHI/EcoRI
TAGGTTTgaattcAGCATATTGGAATGAAGC
    MOMP-VD3CTTTGTGGggatccGGATGTGCAACTTTAGGpGEX-2T, BamHI/EcoRI
TCGTATCgaattcAAAGCTTGCTCGAGACC
    MOMP-VD4AGTTAAAggatccCGAGCAAGCTTTGATGCpGEX-2T, BamHI/EcoRI
TTAGAAGCGgaattcTGCATTTACGTGAGC
  • a Inserted restriction sites are indicated in lowercase letters. Chlamydial gene sequences (in boldface) are derived from C. pneumoniae CWL-029 and C. trachomatis serovar D, respectively.