TABLE 5.

MB-PCR SCCmec typing results for staphylococcal isolates (n = 71)

SpeciesMB-PCR resultaNo. of isolates
mecAccr typebmec classSCCmec
S. epidermidis+3, CAIII6
+3AIII4
+2BIV4
+2AII1
+2, 3, 4ANT2
+2, 4ANT1
+NT1
2, 4Unk1
2, 4, CUnk1
4
S. haemolyticus+CC2V4
+2BIV1
+NT1
4Unk1
S. capitis+2AII4
+3, 4, CANT1
1
S. hominis+3, 4, CANT1
+3, 4ANT3
S. saprophyticusCUnk1
BUnk1
3
S. lugdunensis3Unk1
3
S. simulans4Unk2
2
S. lentus+ANT1
3Unk1
S. xylosus5
S. pseudintermedius2
S. sciuri2
S. warneri2
S. gallinarum1
S. succinus1
S. aureus subsp. anaerobius1
  • a The ccr type and mec class are given according to the nomenclature proposed by Chongtrakool et al. (9). NT, nontypeable SCCmec; Unk, unknown SCC structure; +, mecA positive; −, not detected.

  • b To prevent misclassification, singleplex PCRs using ccrB1- and ccrB3-specific primers in combination with the ccrB1/3 beacon were conducted separately to confirm multiplex MB-PCR results.