TABLE 3.

Analysis of DNA sequences of strains identified incorrectly using database of Microseq 500 16S rDNA bacterial identification system

Strain no.Identification by conventional 16S rRNA gene sequencingIdentification by DNA sequencing of first 527-bp fragment of 16S rRNA gene
Analysis by using database of MicroSeq 500 16S rDNA bacterial identification systemAnalysis by using database of GenBank
Best matchNo. of base differences (%) between strain and best matchSecond best matchNo. of base differences (%) between strain and second best match
8 Granulicatella adiacens Abiotrophia defectiva Granulicatella adiacens 0 (0) Abiotrophia paraadiacens 1 (0.2)
12 Helcococcus kunzii Clostridium hastiforme Helcococcus kunzii 10 (1.9) Sedimentibacter hydroxybenzoicus 91 (17.3)
17 Actinomyces odontolyticus Actinomyces meyeri Actinomyces odontolyticus 0 (0) Actinomyces meyeri 4 (0.8)
19 Olsenella uli Atopobium rimae Olsenella uli 0 (0) Olsenella profusa 21 (4.0)
22 Leptotrichia buccalis Fusobacterium mortiferum Leptotrichia buccalis 2 (0.4) Streptobacillus moniliformis 73 (13.6)
27 Bergeyella zoohelcum Riemerella anatipestifer Bergeyella zoohelcum 2 (0.4) Riemerella anatipestifer 22 (4.2)
32 Arcobacter cryaerophilus Arcobacter butzleri Arcobacter cryaerophilus 0 (0) Arcobacter butzleri 12 (2.3)