TABLE 4.

SCCmec MB-PCR results for MRSA and MSSA strains from various collections

Source or studyGeographic originYr of isolationGenotypic backgroundaMB-PCR resultbNo. (%) of isolates
mecAccr typemec classSCCmec
Kondo et al., 2007 (40) (n = 68)dCanada, Denmark, Egypt, Ireland, Switzerland, United Kingdom, United States1960-1993CC5+1BI1 (1.5)
+2AII22 (32.4)
+2BIV2 (2.9)
CC8+1BI13 (19.1)
+1, CBIc1 (1.5)
+2AII5 (7.4)
+3AIII2 (2.9)
+3, CAIII6 (8.8)
+2BIV2 (2.9)
CC25+2AII1 (1.5)
CC30+2AII3 (4.4)
+3, CAIII1 (1.5)
+NT1 (1.5)
CC45+2AII1 (1.5)
CC59+3AIII1 (1.5)
CC88+2AII1 (1.5)
CC121+1BI1 (1.5)
Other+3AIII1 (1.5)
+3, CAIII1 (1.5)
+2BIV1 (1.5)
+ANT1 (1.5)
Canada (n = 35)Canada2007-2009CC5+2AII5 (14.3)
+2BIV5 (14.3)
+4BVI1 (2.9)
CC8+3, CAIII1 (2.9)
+2BIV12 (34.3)
+CC2V1 (2.9)
+4AVIII1 (2.9)
CC12+CC2V1 (2.9)
CC22+2BIV1 (2.9)
CC30+2BIV1 (2.9)
+CC2V1 (2.9)
CC45+2BIV2 (5.7)
CC59+CC2V2 (5.7)
CC80+2BIV1 (2.9)
NARSA MRSA (n = 66)dBelgium, Brazil, France, Japan, Oman, South Korea, Switzerland, United Kingdom, United States1935-2004CC1+2BIV5 (7.6)
CC5+2AII21 (31.8)
+2BIV1 (1.5)
CC8+1BI11 (16.7)
+2AII1 (1.5)
+3, CAIII4 (6.1)
+2BIV14 (21.2)
CC22+2BIV1 (1.5)
CC30+2AII3 (4.5)
CC45+2AII2 (3.0)
CC59+2BIV1 (1.5)
CC80+2BIV1 (1.5)
CC88+2BIV1 (1.5)
NARSA MSSA (n = 135)CC11e2 (1.5)
2 (1.5)
CC514 (10.4)
CC834 (25.2)
CC155 (3.7)
CC228 (5.9)
CC3028 (20.7)
CC455 (3.7)
CC591 (0.7)
CC9722 (1.5)
1 (0.7)
CC12117 (12.6)
Otherf16 (11.9)
BARG (n = 270)United States (NYC)1996CC5+2AII130 (48.1)
+2BIV10 (3.7)
+2, 4ANT1 (0.4)
CC8+2BIV44 (16.3)
+3, CAIII28 (10.4)
+3AIII1 (0.4)
+1BI16 (5.9)
+NT1 (0.4)
CC30+2AII20 (7.4)
+2, 4ANT1 (0.4)
CC45+2AII4 (1.5)
Other+2AII2 (0.7)
+3AIII1 (0.4)
+2BIV5 (1.9)
+NT1 (0.4)
MSSA5 (1.9)
NYC outpatients (n = 639)United States (NYC)2004-2006CC1+2BIV23 (3.6)
CC8+2BIV480 (75.1)
+2AII1 (0.2)
+4AVIII2 (0.3)
CC5+2AII91 (14.2)
+2BIV23 (3.6)
CC30+2AII4 (0.6)
+2BIV2 (0.3)
CC45+2AII1 (0.2)
+2BIV2 (0.3)
CC59+2BIV8 (1.3)
CC80+2BIV1 (0.2)
CC88+2BIV1 (0.2)
NYC inpatients (n = 573)United States (NYC)2007-2008CC5+2BIV111 (19.4)
+2AII361 (63.0)
+NT2 (0.3)
CC8+2BIV61 (10.6)
+2AII1 (0.2)
+CC2V1 (0.2)
+4AVIII1 (0.2)
CC22+2BIV1 (0.2)
CC30+2AII13 (2.3)
CC45+2AII18 (3.1)
CC59+2BIV1 (0.2)
+NT1 (0.2)
CC398+NT1 (0.2)
  • a Expressed in terms of the MLST CC; except for the NARSA strains, all CCs were inferred by spa repeat pattern analysis (51, 73).

  • b The ccr type and mec class are given according to the nomenclature proposed by Chongtrakool et al. (9). NT, nontypeable; +, mecA positive; −, not detected.

  • c The structure is similar to that of BK793; this isolate possesses an SCCHg region, as reported previously (40).

  • d Not including strains already listed in Table 3.

  • e To prevent misclassification, singleplex PCRs using ccrB1- and ccrB3-specific primers in combination with the ccrB1/3 beacon were conducted separately to confirm the multiplex MB-PCR results.

  • f “Other” NARSA MSSA strains include STs 10, 12, 25, 109, 395, 398, 707, 846, and 890.