TABLE 3.

Representative collection of MRSA strains used for MB-PCR validation

Isolate IDaGeographic originYr of isolationGenotypic backgroundSCCmec type (previous)MB-PCR SCCmec typing resultbReference
CCSTAllelic profile (MLST)spa-type (Ridom)spa repeat pattern (eGenomics)mecA (control)ccrB2 (type 2 ccr)mecI (class A mec)IS1272J (class B mec)ccrC (type 5 ccr)ccrB1/3 (type 1/3 ccr)ccrB4 (type 4 ccr)mecC2 (class C2 mec)SCCmec (this study)
NCTC10442United Kingdom196182503-3-1-1-4-4-16t008YHGFMBQBLOI+++I29
HAR21Finland2002551-4-1-4-12-1-10t001TO2MBMDMGMKI+++I59
HAR40Belgium199552281-4-1-4-12-24-29t001TO2MBMDMGMKI+++I59
E2125Denmark196482473-3-1-12-4-4-16t051YHFGFMBQBLOI+++I61
COLUnited Kingdom196082503-3-1-1-4-4-16t051YHFGFMBQBLOI+++I61
UK13136United Kingdom196582503-3-1-1-4-4-16t2481YHFGFMBBLOI+++I61
BK793Egypt196182503-3-1-1-4-4-16t008YHGFMBQBLOI++++I61
HPV107Portugal199282473-3-1-12-4-4-16t051YHFGFMBQBLOIA+++I61
BK1953United States199582473-3-1-12-4-4-16t051YHFGFMBQBLOIA+++I61
PER88Spain199282473-3-1-12-4-4-16t051YHFGFMBQBLOIA+++I61
PER184Spain199182473-3-1-12-4-4-16t121YHFMBQBLOIA+++I61
PER 34Spain198982503-3-1-1-4-4-16t051YHFGFMBQBLOIA+++I61
PL72Poland1991551-4-1-4-12-1-10t053TJMBMDMGMBI-var+++I59
POL3Poland1992551-4-1-4-12-1-10t053TJMBMDMGMBI-var+++I59
N315Japan1982551-4-1-4-12-1-10t002TJMBMDMGMKII+++II30
Mu3Japan1997551-4-1-4-12-1-10t002TJMBMDMGMKII+++II25
Mu50Japan1997551-4-1-4-12-1-10t002TJMBMDMGMKII+++II41
BK2464United States1996551-4-1-4-12-1-10t002TJMBMDMGMKII+++II57
MRSA252United Kingdom199730362-2-2-2-3-3-2t018WGKAKAOMQQQII+++II27
BK351Ireland1991883-3-1-1-4-4-3t190YMBQBLOIIA+++II40
JCSC3063Japan2001-200291c891-26-28-18-18-33-50t375Y2EJCMBPBIIb+++II26
RN7170Canada1989551-4-1-4-12-1-10t002TJMBMDMGMKIIE+++II40
85/2082New Zealand198582392-3-1-1-4-4-3t037WGKAOMQIII++++III29
ANS46Australia198282392-3-1-1-4-4-3t037WGKAOMQIII++++III61
R35United States198782392-3-1-1-4-4-3t037WGKAOMQIII++++III61
LHH1United States199482392-3-1-1-4-4-3t037WGKAOMQIII++++III61
BK2421United States199682392-3-1-1-4-4-3t037WGKAOMQIII++++III61
HSJ216Portugal199782392-3-1-1-4-4-3t037WGKAOMQIII++++III61
HU106Hungary199682392-3-1-1-4-4-3t538WGKAOKAOKAOMQIII++++III61
HUSA304Hungary199382392-3-1-1-4-4-3dWKAKAOKAOMQIII++++III61
HU25Brazil199382392-3-1-1-4-4-3t138XKAOMQIIIA++++III61
HDG2Portugal199382392-3-1-1-4-4-3t421WGKAOMIIIB+++III61
DEN907Denmark200182392-3-1-1-4-4-3t037WGKAOMQNTe+++III59
CA05United States19994525610-14-8-6-10- 37-2t040A2AKEMBKBIVa+++IV50
MW2United States1998111-1-1-1-1-1-1t128UJJFKBPEIVa+++IV4
HSA74Portugal1993551-4-1-4-12-1-10t002TJMBMDMGMKIVa+++IV58
FFP311Portugal1996551-4-1-4-12-1-10t311TJMBDMGMKIVa+++IV58
FPR3757United States2003883-3-1-1-4-4-3t008YHGFMBQBLOIVa+++IV13
8/6-3PUnited States199687701-3-1-1-4-4-3t008YHGFMBQBLOIVb+++IV50
JCSC4788Japan200184513-4-1-42-4-4-3t1852YGFMFMBQBLOIVc+++IV37
ARG9Argentina1996551-4-1-4-12-1-10t002TJMBMDMGMKIVc+++IV58
Q2314United States1996551-4-1-4-12-1-10t088TJMBMDMGGMKIVc+++IV1
JCSC4469Japan1982551-4-1-4-12-1-10t002TJMBMDMGMKIVd+++IV49
AR43/3330.1Ireland1988-2002883-3-1-1-4-4-3t190YMBQBLOIVE++++IV68
HAR22Finland200222227-6-1-5-8-8-6t022TJEJNF2MNF2MOMOKRIVh+++IV52
HAR36Greece200282543-12-1-1-4-4-3t009YGFMBQBLQBLPOIVh++++IV52
GRE120Greece1993883-3-1-1-4-4-3t036YGFMBQBLPOIV+++IV2
BARG II 17United States1996883-3-1-1-4-4-3t064YHGCMBQBLOIV+++IV61
WBG10049Australia199930302-2-2-2-6-3-2t019XKAKAOMQIV+++IV64
COB3Colombia1996551-4-1-4-12-1-10t1107TMDMGKIVNT+++IV61
WISAustralia454510-14-8-6-10- 37-2t123A2AKBEKBKBV+++V31
HT0826France200315237746-75-49-50-13-68-60t355UJ2GMKKPNSGV+++V53
SW31.1United States20083983983-35-19-2-20-26-39t034XKAOAOBQV+++V71
SW62.1United States20083983983-35-19-2-20-26-39t034XKAOAOBQV+++V71
SW181.1United States20083983983-35-19-2-20-26-39t034XKAOAOBQV+++V71
HU01010TUnited States20083983983-35-19-2-20-26-39t034XKAOAOBQV+++V71
HU01011NUnited States20083983983-35-19-2-20-26-39t034XKAOAOBQV+++V71
HDE288Portugal1996551-4-1-4-12-1-10t311TJMBDMGMKVI+++VI58
IPO92Portugal2001551-4-1-4-12-1-10t311TJMBDMGMKVI+++VI58
  • a Prototype strains used to optimize the MB-PCR assay are in boldface.

  • b +, detected; −, not detected.

  • c ST89 is currently assigned to CC509 by eBURST, version 3 (17).

  • d The spa repeat pattern WKAKAOKAOMQ is not recognized by the Ridom SpaServer as of this writing.

  • e NT, nontypeable.