TABLE 1.

STEC strains used in this study

StrainOriginSerotypeToxinPCR result (band size)Western blot results for eaeA
L (6.2 kb)D (2.6 kb)C (5.5 kb)eaehlyA
S-1DeerO111:H452− (1.9 kb)+
S-2DeerO111:H452− (1.9 kb)+
S-3DeerO111:H452− (1.9 kb)+
S-4DeerOut:H452+
S-5DeerO93:H−2C+
S-6DeerO96:H−2− (1.9 kb)C+
S-7DeerO96:H−2− (1.9 kb)C+
S-8DeerOut:H252+++
S-9DeerO8:H72− (1.9 kb)
S-10DeerO96:H−2− (1.9 kb)
S-11DeerO96:H−2− (1.9 kb)
HI-1SheepO153:H251− (<1.0 kb)
HI-2SheepO165:H−1, 2− (2.5 kb)C+
HI-5SheepOut:H161
HI-7SheepOut:H−1+4 kb++
HI-11SheepO2:Hut2+3 kb++
HI-NSheepOut:H−1,2++
K-7SeagullO136:H162++− (0.6 kb)+
K-10SeagullO153:H−1+
fly-11FlyONT:H−1, 2+
F-16FlyO157:H71, 2+++γ++
F-156FlyO157:H71, 2+++γ++
F-158FlyO157:H71, 2+++γ++
F2-1FlyO157:H7+++γ++
Obi-1*HumanO157:H72+++γ++
SakaiHumanO157:H71, 2+++γ++
T-1*HumanO157:H−1+++γ++
T-46*HumanO157:H71, 2+++γ++
K-159*HumanO157:H72+++γ++
K-262*HumanO157:H71, 2+++γ++
EC097028*HumanO157:H7+++γ++
SRSalmon roeO157:H71, 2+++γ++
AS16CattleO157:H72++γ++
AS17CattleO157:H72+++γ++
AS18CattleO157:H72++γ++
AS19CattleO157:H72++γ++
AS20CattleO157:H71, 2++γ++
KT1CattleO157:H72++γ++
HK1CattleO157:H72+++γ++
MB1CattleO157:H71+++γ+
TS−CattleO157:H72+++γ++
IK3CattleO157:H72+++γ++
KR1CattleO1191, 2+β++
IK4CattleO1191β++
AS1*HumanO26:H111, 2+β++
AS15HumanO26:H111, 2+β++
H4-52CattleO26:H−1β++
H4-54CattleO26:H111β+
H5-7CattleO26:H−1β+
H5-16*CattleO26:H−1β+
H5-26CattleO26:H111+β+
H5-160CattleO26:H111β+
H7-10CattleO26:H111β++
H8-55CattleO26:H111+β++
H8-87CattleO26:H111+β+
IK2CattleO261+β++
  • a Symbols and abbreviations: *, seven STEC strains used for Southern hybridization; −, negative; +, positive; C, eae common primer positive but nontypeable; γ, eae; γ-specific primer positive; β, eae β-specific primer positive.