TABLE 2.

Compilation of RDs between F. tularensis subsp. tularensis and non-Japanese F. tularensis subsp. holarctica strains

RDaSize (bp)Open reading frameHomolog (GenBank accession no.)E valuebDirect repeat motife
RD1598Hypothetical proteincAgAATGGATTCTACGGTCAAGCCGTAGAATGA
AaAATGGATTCTACGGTCAAGCCGTAGAATGA
RD2975Oligopeptide ABC transporter, ATP-binding protein, oppDNP_230739.12 E-96CATCCGTATACAAAAGtcCTtCTAAATgCtATtCCTA CATCCGTATACAAAAGgtCTgCTAAATaCaATgCCTA
Oligopeptide ABC transporter, ATP-binding protein, oppFNP_439277.11 E-88
RD31,731Putative modulator of drug activity B, mdaBNP_289602.12 E-72TATCTAAtGAATGGGAGCTA-TC
TA-CTAAa-AATGG-AG-TAATC
Conserved hypothetical proteinNP_436326.16 E-29
Hypothetical arginine-ornithine operon transport proteinNP_249582.11 E-09
RD41,314Piperideine-6-carboxylate dehydrogenase (strongly homologous to aldehyde de- hydrogenases)BAB19801.11 E-119TCgGCTTTTAAC TCaGCTTTTAAC
Conserved hypothetical proteinNP_232243.16 E-55
RD51,117Adenine-specific DNA methyltransferaseNP_478077.16 E-17AGtAgAAGAGCAAgAGCAAtATaAAActG
AGcAcAAGAGCAAaAGCAAgATaAAAaaG
Type I restriction-modification system, subunitNP_345026.11 E-11
RD64,260Conserved hypothetical proteinNP_488560.12 E-41GtTATGTGCTT
GcTATGTGCTT
Hypothetical protein
RD71,458Aspartokinase I, homoserine dehydrogenase INP_414543.11 E-77GcTAgCAAAATTAG
GaTAtCAAAA-TAG
Homoserine kinase (possibly truncated)NP_299503.13 E-18
RD8∼11,500Snf2 family ATP-dependent helicase, HepANP_603318.10.0Insertion sequence elementsd
Type III restriction-modification system, subunit (possibly truncated)NP_603321.11 E-133
Antirestriction proteinNP_478454.14 E-25
Hypothetical proteinZP-00044100.11 E-10
Hypothetical proteinNP_282940.17 E-09
MobC-like proteinCAB62410.16 E-08
Hypothetical proteinZP_00086265.14 E-04
  • a All RDs except RD8 were confirmed by PCR analysis and sequencing of the F. tularensis subsp. holarctica LVS FSC 155.

  • b E values were obtained from NCBI Blast2 searches for sequences in the GenBank database by using the low-complexity filter option.

  • c Open reading frame A in Fig. 2.

  • d GenBank accession no. AF357005.

  • e Lowercase letters indicate deviation from the consensus sequence.