TABLE 3.

Variation in Ct for different copy numbers of HPV types in reactions 1 and 2a

Reaction and HPV typeMean Ct ± SD of (no. of HPV genomes):
106105104103102
Reaction 1
    HPV1617.75 ± 0.4121.04 ± 0.5024.65 ± 0.5227.85 ± 0.8131.35 ± 0.95
    HPV3118.78 ± 0.7722.87 ± 1.6426.55 ± 0.6029.18 ± 0.8433.73 ± 0.71
    HPV1818.69 ± 0.4622.09 ± 0.8225.47 ± 0.5728.78 ± 0.532.53 ± 0.59
    HPV4519.11 ± 0.6422.15 ± 0.7525.96 ± 0.8429.23 ± 0.6733.02 ± 0.57
Reaction 2
    HPV3319.47 ± 0.5422.30 ± 0.5525.78 ± 0.8729.02 ± 0.64
    HPV3520.88 ± 0.7924.67 ± 0.9427.69 ± 1.0430.70 ± 1.00
    HPV3920.07 ± 1.2723.94 ± 0.7726.87b ± 1.3830.55 ± 1.02
    HPV5220.48 ± 0.5824.10 ± 0.6027.90 ± 0.8830.84 ± 0.53
    HPV5820.85 ± 0.6224.12 ± 0.8227.58 ± 0.9430.44 ± 0.59
    HPV6718.89 ± 0.6722.22 ± 0.5725.55 ± 0.8627.93 ± 1.07
  • a Values are based on 12 independent measurements at each copy number.

  • b Based on 11 independent measurements.