TABLE 1.

Amplification primers and molecular beacon probes used in this study

OligonucleotideSequencePositiona
Primers
    grlA205 (Forward)5′-GAT GTT ATT GGT CAA TAT CAT CCA-3′205-228
    grlA396 (Reverse)5′-AAG AAA CTG TCT CTT TAT TAA TAT CAC GT-3′396-424
Probesb
    S80-FAM5′ (FAM)-CAG CGT ACA CTG AGG AGT CTC CGC TG-3′ (DABCYL)233-248
    E84-FAM5′ (FAM)-CGC GAA GTG TAC GAA GCA ATG GTC GCG-3′ (DABCYL)243-259
    Sau-TET5′ (TET)-CCG GGT ATC GAT AAT GAT CCG CCA GCC CGG-3′ (DABCYL)324-343
  • a Nucleotide positions in the grlA gene (start codon, 1 to 3) from GenBank accession no. D67075 .

  • b The underlined sequences constitute the stem of each molecular beacon. FAM, 6-carboxylfluorescein; TET, tetrachloro-6-carboxylfluorescein.