TABLE 1.

Analytical sensitivity of four extraction methodsa

Extraction methodNo. positive/total no. tested at a viral input (PFU/ml) of:
10010−110−210−3
TRIzol8/94/90/90/9
QIAamp17/1817/184/17∗0/17∗
BioRobot M4818/1815/182/180/18
MagNA Pure18/1812/180/17∗0/17∗
  • a Evaluation of TRIzol was discontinued after nine replicates. ∗, A single replicate was excluded from analysis because bands on autoradiographs were smeared and not interpretable.