TABLE 1.

Primer sequences

PrimerOligonucleotide sequence (5′-3′) (no. of nucleotides)aTarget sequence, amplicon size (bp)PurposeReference(s) or source
SRV3-1CGG YCC AGA CTC CTA CGG G (19)16S rDNA 330-533, ∼200Bacterial identification by PCR and sequencing12
SRV3-2TTA CCG CGG CTG CTG GCA C (19)
gyrPA-398CCT GAC CAT CCG TCG CCA CAA C (22) P. aeruginosa gyrB 398-620, 222 P. aeruginosa-specific PCRThis study
gyrPA-620CGC AGC AGG ATG CCG ACG CC (20)
ETA-1GAC AAC GCC CTC AGC ATC ACC AGC (24) P. aeruginosa ETA, 367 P. aeruginosa-specific PCR9, 17
ETA-2CGC TGG CCC ATT CGC TCC AGC GCT (24)
VIC-1TTC CCT CGC AGA GAA AAC ATC (26) P. aeruginosa algD encoding GDP mannose dehydrogenase 520 P. aeruginosa-specific PCR2, 19
VIC-2CCT GGT TGA TCA GGT CGA TCT (21)
OPR-1GCT CTG GCT CTG GCT GCT (18) P. aeruginosa oprl, encoding surface lipoprotein 1, 330 P. aeruginosa-specific PCR3, 4
OPR-2AGG GCA CGC TCG TTA GCC (18)
  • a Y = C or T.