TABLE 2.

Comparison of GeneScan RT-PCR with culture and DIF

Culture or DIF resultNo. of specimans with RT-PCR assay resulta
HRSV +HPIV1 +HPIV2 +HPIV3 +Flu A +Flu B +Negative
HRSV +
    Culture41000003
    DIF25000000
HPIV1 +
    Culture0000000
    DIF0000000
HPIV2 +
    Culture0020000
    DIF0000000
HPIV3 +
    Culture0005003c
    DIF0000000
Flu A +
    Culture0000301f
    DIF0000000
Flu B +
    Culture00000100
    DIF0000000
Negative
    Culture75b3c24d6be1250
    DIF201013312
  • a Shown are numbers of specimens that tested positive or negative for the respective respiratory viruses by RT-PCR assay culture and DIF. A total of 470 specimens were tested by RT-PCR, 405 were tested by culture, and 65 were treated by DIF. Flu, influenza virus.

  • b HRSV and influenza A virus were identified by RT-PCR in one culture negative specimen.

  • c HRSV was identified by culture and RT-PCR and HPIV3 was identified by culture only in one specimen.

  • d HRSV was identified by culture and RT-PCR and HPIV3 was identified by RT-PCR in one specimen.

  • e HPIV1 and influenza A virus were identified by RT-PCR in one specimen.

  • f Influenza A virus could not be confirmed by culture passage.