TABLE 3.

sopE1 polymorphism among SPV and EPV strains of S. enterica serotype Paratyphi B and other serotypes and lysogens of S. enterica strains

StrainSerotypePathovarsopE1 PCR resultsopE1 RFLP patternasopE1 (I) PCR resultbsopE1 (II) PCR resultc
Upstream to orfRDownstream to orf45Upstream to orfRDownstream to orf194
Tester strains (lysogen)
    B309Paratyphi BSPV+II++
    00-08652Paratyphi BEPV
    00-08652 (ΦSopE309)dParatyphi BEPV+II++
    A36Typhimurium
    A36 (SopEΦ)eTyphimurium+I+g
    A36 (ΦSopE309)fTyphimurium+II++
Clinical strains
    99-06072Paratyphi BSPV+II++
    99-02820Paratyphi BSPV+II++
    99-08309Paratyphi BSPV+II++
    99-06148Paratyphi BSPV+II++
    97-15877Paratyphi BSPV+II++
    SAR41Paratyphi BSPV+II++
    99-08380Paratyphi BSPV+I++
    96-01098Paratyphi BEPVh+I++
    97-12134Paratyphi BEPVh+I++
    99-01096Paratyphi BEPVh+I++
    99-01097Paratyphi BEPVh+I++
    99-08163Paratyphi BEPVh+I
    99-04814Paratyphi BEPVh+I++
    96-08640Typhi+I++
    RKI130Heidelberg+I++
    98-11635Typhimurium DT68i+I++
    93-00080Typhimurium DT204i+I++
    75-01646Typhimurium DT175i+I++
    76-E8Enteritidis+II++
    63-00647Gallinarum+II++
    99-03279Hadar+II++
    2229Dublin+II++
  • a See Fig. 3 and reference 21.

  • b Cluster I sopE1 PCR (17).

  • c Cluster II sopE1 PCR (17).

  • d Lysogenization of 00-08652 (EPV phage-free tester strain) with ΦSopE309 (isolated from SPV S. enterica serotype Paratyphi B strain B 309).

  • e Lysogenization of the S. enterica serotype Typhimurium phage-free tester strain A36 with the sopE1-carrying bacteriophage SopEΦ isolated from clinical S. enterica serotype Typhimurium strains (17).

  • f Lysogenization of the S. enterica serotype Typhimurium phage-free tester strain A36 with the sopE1-carrying bacteriophage ΦSopE309 isolated from systemic pathovar of S. enterica serotype Paratyphi B strain B 309.

  • g The PCR is negative because a kanamycin resistance gene cassette has been introduced (17).

  • h Enteric pathovar carrying the sopE1 determinant.

  • i DT, definitive phage type.