TABLE 2.

Cross-reactivity of WNV NS5 and E protein with DEN patient sera

SampleaMFITiter
NS5bE proteincE protein MIAdHI
1A1,225280<10010
1B1,3682,016200160
2A2,3251,44010020
2B2,6142,95140080
3A5,6776,58725,60010,240
3B2,4724,8943,200320
4A1,348180<100
4B5,7501,554200640
5A674235<100
5B7151,49720040
6A810113<100
6B9531,081100160
7A2,432289<100
7B4,9352,86010080
8A720875<10020
8B829558<10080
9A8643,459400160
9B1,8644,8261600160
10A1,8321,36610020
10B1,7556,6866,40010,240
11A4,6587,47451,20010,240
11B1,7235,0136,4001,280
12A8415,3443200640
12B7946,10512,8002,560
13A3,83382510080
13B2,7611,54980080
14A6785,57864002,560
14B7574,720160080
15A1,5484,8071600160
15B1,5878,62651,20010,240
16A9456,1593,200640
16B1,1286,4176,40080
17A1,427225<100
17B1,5543,108800800
  • a Seventeen pairs of acute-phase (A) and convalescent-phase (B) sera from DEN-infected individuals were tested.

  • b The cutoff for positivity for NS5 is 4,366. There were 3 positive samples out of 34 (8.8%).

  • c The cutoff for positivity for E protein is 1,084 (Wong et al., submitted). There were 24 positive samples out of 34 (71%).

  • d E protein MIA titers represent the maximal dilutions of patient sera that were reactive in the E-protein-based MIA above the MFI cutoff of 1,084.