TABLE 2.

Agreement between real-time PCR results and molecular identificationa

Organism group (n)b16S-V3 sequencing result (n)gyrB PCR result (n)gyrB sequencing result (n)No. (%) of isolates positive by:
VIC PCRETA PCROPR PCR
E1 (25)NDc P. aeruginosa (25) ND23 (92)24 (96)25 (100)
E2 (25)ND P. aeruginosa (25)ND22 (88)25 (100)25 (100)
H1 (50)ND P. aeruginosa (50)ND46 (92)48 (96)50 (100)
H2 (50) P. aeruginosa group (12) P. aeruginosa (12) P. aeruginosa (12)10 (83)11 (92)12 (100)
Other GNR (38)Not P. aeruginosa (38)ND001 (3)
H3 (50) P. aeruginosa group (1) P. aeruginosa (1) P. aeruginosa (1)000
Other GNRd (49)Not P. aeruginosa (49)ND000
Controls (4)Other GNR (4)Not P. aeruginosa (4)ND000
  • a For all discrepancies between VIC, ETA, and OPR real-time PCR results, 16S-V3 sequencing was performed to verify that isolates were in the P. aeruginosa group.

  • b n, number of isolates.

  • c ND, not routinely done.

  • d GNR, gram-negative rod.