TABLE 1.

Comparison of four different assays for HIV-1 RNAa

No. of copies/ml predicted by VQA (control)No. of copies/ml
AMPLICOR ultrasensitive assayModified AMPLICOR ultrasensitive assaySingle-copy assay
Run 1Run 2Run 3Run 4MeanSDRun 1Run 2Run 3Run 4MeanSDRun 1Run 2Run 3Run 4MeanSD
200173176170127162231901491401991702918720018923220230
10090137150901173187451078882268584751399629
50247060595320692761445019323339624214
253128NegbNeg302.127182817235.815251130208.8
12.5NegNegNegNegNegNeg9111511122.51181012101.7
6.25NegNegNegNegNegNeg171257105.429886.83.2
3.125NegNegNegNegNegNeg10NegNegNeg10025433.51.3
1.56NegNegNegNegNegNegNegNegNegNegNegNeg21162.52.4
0.78NegNegNegNegNegNegNegNegNegNegNegNeg0.310.8NDc0.70.4
  • a An amount of preassayed virus with the corresponding number of copies of RNA indicated was mixed with normal plasma.

  • b Neg, the OD was not above background.

  • c ND, not determined (due to RCAS value <15,000 copies/reaction mixture).