Table 3.

All specimens providing positive results in the PPNG-PCR2 assayd

Specimen (type; yr of isolation)PhenotypeaPPNG-PCR2 result (CT value)porA PCR result (CT value)ΔCT valueNG-MASTPlasmid typeb
N. gonorrhoeae
    1 (Iso; 2004)PPNGPositive (21)Positive (25)+4211
    2 (Iso; 2004)PPNGPositive (25)Positive (29)+4211
    3 (Iso; 2004)PPNGPositive (26)Positive (28)+2211
    4 (Iso; 2005)PPNGPositive (31)Positive (34)+3421
    5 (Iso; 2005)PPNGPositive (26)Positive (30)+41496
    6 (Iso; 2005)PPNGPositive (34)Positive (38)+41797
    7 (Iso; 2005)PPNGPositive (24)Positive (27)+31919
    8 (Iso; 2005)PPNGPositive (24)Positive (27)+31902
    9 (Iso; 2005)PPNGPositive (31)Positive (34)+31921
    10 (Iso; 2005)PPNGPositive (32)Positive (34)+21922
    11 (Iso; 2002)PPNGPositive (13)Positive (17)+4NPAfrican
    12 (Iso; 2004)PPNGPositive (14)Positive (18)+4NPAfrican
    13 (Iso; 2004)PPNGPositive (15)Positive (18)+3NPAfrican
    14 (Iso; 2004)PPNGPositive (15)Positive (19)+4NPAfrican
    15 (Iso; 2001)PPNGPositive (12)Positive (15)+3NPAsian
    16 (Iso; 2002)PPNGPositive (13)Positive (17)+4NPAsian
    17 (Iso; 2002)PPNGPositive (14)Positive (16)+2NPAsian
    18 (Iso; 2004)PPNGPositive (14)Positive (17)+3NPAsian
    19 (Iso; 2004)PPNGPositive (14)Positive (17)+3NPAsian
    20 (Iso; 2002)PPNGPositive (15)Positive (16)+1NPAsian
    21 (Iso; 2001)PPNGPositive (15)Positive (18)+3NPAsian
    22 (Iso; 2004)PPNGPositive (18)Positive (20)+2NPAsian
    23 (Iso; 2004)PPNGPositive (13)Positive (17)+4NPRio/Toronto
N. gonorrhoeae-negative clinical samples from Queensland (Path-QLD)
    24 (urine; 2007)NAPositive (33)NegativeNot applicable
    25 (urine; 2007)NAPositive (39)NegativeNot applicable
N. gonorrhoeae-negative clinical samples from the Northern Territory (Path-RDH)
    26 (NA; 2008-2009)NAPositive (35)NegativeNot applicable
    27 (NA; 2008-2009)NAPositive (38)NegativeNot applicable
    28 (NA; 2008-2009)NAPositive (39)NegativeNot applicable
    29 (NA; 2008-2009)NAPositive (39)NegativeNot applicable
    30 (NA; 2008-2009)NAPositive (41)NegativeNot applicable
    31 (NA; 2008-2009)NAPositive (43)NegativeNot applicable
N. gonorrhoeae-positive clinical samples from Queensland (Path-QLD)
    32 (UrSw; 2004)PPNGPositive (17)Positive (21)+4798
    33 (UrSw; 2004)PPNGPositive (19)Positive (23)+4New
    34 (urine; 2003)NAPositive (22)Positive (25)+3NP
    35 (urine; 2003)NAPositive (22)Positive (26)+4NP
    36 (PnSw; 2003)NAPositive (22)Positive (26)+4NP
    37 (CxSw; 2003)PPNGPositive (24)Positive (30)+6NP
    38 (urine; 2006)NAPositive (25)Positive (29)+41797
    39 (urine; 2003)NAPositive (28)Positive (33)+5NP
    40 (urine; 2003)NAPositive (43)Positive (29)−14NP
N. gonorrhoeae-positive clinical samples from the Northern Territory (Path-RDH)
    41 (NA; 2008-2009)PPNGPositive (31)Positive (35)+4758
    42 (NA; 2008-2009)PPNGPositive (26)Positive (30)+41405
    43 (NA; 2008-2009)NAPositive (32)Positive (37)+51405
    44 (NA; 2008-2009)PPNGPositive (26)Positive (29)+31554
    45 (NA; 2008-2009)PPNGPositive (21)Positive (26)+51691
    46 (NA; 2008-2009)NAPositive (26)Positive (30)+41691
    47 (NA; 2008-2009)NAPositive (25)Positive (29)+42996
    48 (NA; 2008-2009)NAPositive (29)Positive (32)+42996
    49 (NA; 2008-2009)NAPositive (22)Positive (24)+23782
    50 (NA; 2008-2009)PPNGPositive (26)Positive (30)+43782
    51 (NA; 2008-2009)PPNGPositive (22)Positive (26)+4New
    52 (NA; 2008-2009)PPNGPositive (22)Positive (26)+4New
    53 (NA; 2008-2009)PPNGPositive (25)Positive (31)+6New
    54 (NA; 2008-2009)NAPositive (28)Positive (31)+3NAc
    55 (NA; 2008-2009)NAPositive (31)Positive (36)+5NAc
    56 (NA; 2008-2009)Non-PPNGPositive (39)Positive (31)−8NAc
    57 (NA; 2008-2009)Non-PPNGPositive (40)Positive (31)−9NAc
    58 (NA; 2008-2009)Non-PPNGPositive (40)Positive (32)−8NAc
    59 (NA; 2008-2009)NAPositive (43)Positive (32)−11NAc
  • a The presence of penicillinase was assessed by phenotypic methods.

  • b The plasmid type was available for the 13 Queensland isolates only.

  • c Amplification of the NG-MAST porB sequence, tbpB sequence, or both sequences was unsuccessful for these specimens.

  • d CxSw, cervical swab; PnSw, penile swab; UrSw, urethral swab; Iso, isolate; PPNG, penicillinase-producing N. gonorrhoeae; NA, not available; NP, not performed; New, a matching sequence type could not be assigned by the NG-MAST database, and the isolate was considered to represent previously unidentified alleles or combinations of alleles.