TABLE 3.

Comparison of spectrophotometric methodology to QRT PCR for quantification of PCR ampliconsa

AdV typeInput PCR amplicon copy no.bQRT PCR copy no.cMean copy no.SDCV
165.76345.77500.01540.0027
5.7686
5.7924
265.90315.910310.02510.0042
5.8887
5.9375
465.60215.59480.03420.0061
5.6232
5.5563
565.90045.86370.03330.0057
5.8370
5.8513
665.86925.84760.02800.0048
5.8156
5.8563
765.65325.65320.03870.0068
5.6902
5.6128
965.88085.82930.04770.0082
5.7889
5.8129
1065.57985.56030.01820.0033
5.5441
5.5563
1165.89215.86730.06880.0117
5.7853
5.9140
1265.53155.49600.03470.0063
5.4914
5.4624
1765.75595.75910.02700.0047
5.7868
5.7328
1865.43145.44720.06990.0128
5.5185
5.3802
2865.80625.77790.04920.0085
5.7193
5.8028
3165.38025.47230.08240.0151
5.4771
5.5441
3765.75595.78490.04040.0070
5.8299
5.7649
Avg5.70930.0073
  • a All data are expressed in log10 units.

  • b Based on spectrophotometric methodology.

  • c Mean copy number of each of three QRT-PCR runs.